How to Prepare a Schiff's Reagent

• Dissolve 5g of basic fuchsin in 900ml of boiling distilled water
• Cool to approximately 50^oC and slowly add 100ml of 1N HCl
• Cool to approximately 25^oC and dissolve 10g of K₂S₂O₅
• Shake for 3 minutes and incubate in the dark at room temperature for 24 hours
• Add 5 grams of fine activated charcoal and shake for 3 minutes
• Filter solution (should be clear)
• Store at 4^oC in a foil covered bottle

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